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Image Search Results
Journal: JCI insight
Article Title: PCYT2 inhibits epithelial-to-mesenchymal transition in colorectal cancer by elevating YAP1 phosphorylation.
doi: 10.1172/jci.insight.178823
Figure Lengend Snippet: Figure 4. PCYT2 affects the phosphorylation levels of YAP1 and changes the proportion of YAP1 in 778
Article Snippet: Cell extracts were 520 prepared by mechanical sonication, and protein–DNA complexes were immunoprecipitated 521 using
Techniques: Phospho-proteomics
Journal: JCI insight
Article Title: PCYT2 inhibits epithelial-to-mesenchymal transition in colorectal cancer by elevating YAP1 phosphorylation.
doi: 10.1172/jci.insight.178823
Figure Lengend Snippet: Figure 5. PCYT2 regulates EMT in colorectal cancer via YAP1 translocation induced by PEBP1. (A 802
Article Snippet: Cell extracts were 520 prepared by mechanical sonication, and protein–DNA complexes were immunoprecipitated 521 using
Techniques: Translocation Assay
Journal: JCI insight
Article Title: PCYT2 inhibits epithelial-to-mesenchymal transition in colorectal cancer by elevating YAP1 phosphorylation.
doi: 10.1172/jci.insight.178823
Figure Lengend Snippet: Figure 6. PPP2R1A interacts with YAP1 and PEBP1. (A) Detection of the interaction between PEBP1 820
Article Snippet: Cell extracts were 520 prepared by mechanical sonication, and protein–DNA complexes were immunoprecipitated 521 using
Techniques:
Journal: Developmental cell
Article Title: Yorkie growth promoting activity is limited by Atg1-mediated phosphorylation
doi: 10.1016/j.devcel.2020.01.011
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Recombinant, SYBR Green Assay, Plasmid Preparation, Protease Inhibitor, Transfection, Chromatography, Electron Microscopy, Membrane, Reverse Transcription, Software
Journal: Translational Lung Cancer Research
Article Title: Targeting PKCι-PAK1 in EGFR-mutation positive non-small cell lung cancer
doi: 10.21037/tlcr.2019.08.25
Figure Lengend Snippet: Antibodies used in the study
Article Snippet:
Techniques:
Journal: bioRxiv
Article Title: The EGF/EGFR-YAP1/TEAD2 axis-mediated BRAF inhibitor vemurafenib resistance upregulates STIM1 in melanoma
doi: 10.1101/2024.01.29.577717
Figure Lengend Snippet: BRAF inhibition increase STIM1 expression through YAP1 A. WB analysis of p-ERK level in control and vemurafenib resistant WM793 cells B. WB analysis of p-STAT3 level in control and vemurafenib resistant WM793 cells C. WB analysis of the effect of STAT3 shRNA on STIM1 level D. WB analysis of β-catenin level in control and vemurafenib resistant WM793 cells E. WB analysis of the effect of β -catenin shRNA on STIM1 level F. WB analysis of YAP1 and TAZ level in control and vemurafenib resistant WM793 cells G. The effect of Braf resistance on YAP1 nucleus localization H. WB analysis of the effect of YAP1 shRNA on STIM1 level I. Q-PCR analysis of the effect of YAP1 shRNA on STIM1 level J. WB analysis of the effect of YAP1 overexpression on STIM1 level
Article Snippet: The following antibodies were used in this study: mouse monoclonal GAPDH(G8795; Sigma-Aldrich, Shanghai, China), mouse monoclonal STIM1(sc-166840; Santa Cruz, Shanghai, China), rabbit polyclonal Actin(sc-1616; Santa Cruz, Shanghai, China), mouse monoclonal Raf-B(sc-5284; Santa Cruz, Shanghai, China), mouse monoclonal EGFR(sc-373746; Santa Cruz, Shanghai, China), mouse monoclonal GAPDH(sc-32233; Santa Cruz, Shanghai, China), mouse monoclonal p-ERK(sc-7383; Santa Cruz, Shanghai, China), mouse monoclonal ERK(sc-514302; Santa Cruz, Shanghai, China), mouse monoclonal p-stat3(sc-8059; Santa Cruz, Shanghai, China), rabbit polyclonal β-catenin(A01211-40; Genscript, China),
Techniques: Inhibition, Expressing, shRNA, Over Expression
Journal: bioRxiv
Article Title: The EGF/EGFR-YAP1/TEAD2 axis-mediated BRAF inhibitor vemurafenib resistance upregulates STIM1 in melanoma
doi: 10.1101/2024.01.29.577717
Figure Lengend Snippet: YAP1/TEAD2 directly regulate Vemurafenib resistance induced STIM1 upregulation A. WB analysis of the effect of TEAD2 overexpression on STIM1 level. B. Q-PCR analysis of the effect of TEAD2 shRNA efficiency. C. The effect of TEAD2 shRNA on STIM1 mRNA level. D. Motif of TEAD2 transcription factor on the top left, and graphical representation of predicted binding sites. Forecasting tool supplied by JASPAR website ( https://jaspar.genereg.net/ ). E. Luciferase activity of promoter fragments in contrast to pRL-TK in control and YAP1-TEAD2 overexpression cell lines. F. Luciferase activity of the P-900 wild type and mutant in contrast to pRL-TK in control and YAP1-TEAD2 overexpression cell lines. G. CHIP analysis of the binding of YAP1 to the STIM1 promoter in WM793 control and vemurafenib resistant cells. ***p<0.001.
Article Snippet: The following antibodies were used in this study: mouse monoclonal GAPDH(G8795; Sigma-Aldrich, Shanghai, China), mouse monoclonal STIM1(sc-166840; Santa Cruz, Shanghai, China), rabbit polyclonal Actin(sc-1616; Santa Cruz, Shanghai, China), mouse monoclonal Raf-B(sc-5284; Santa Cruz, Shanghai, China), mouse monoclonal EGFR(sc-373746; Santa Cruz, Shanghai, China), mouse monoclonal GAPDH(sc-32233; Santa Cruz, Shanghai, China), mouse monoclonal p-ERK(sc-7383; Santa Cruz, Shanghai, China), mouse monoclonal ERK(sc-514302; Santa Cruz, Shanghai, China), mouse monoclonal p-stat3(sc-8059; Santa Cruz, Shanghai, China), rabbit polyclonal β-catenin(A01211-40; Genscript, China),
Techniques: Over Expression, shRNA, Binding Assay, Luciferase, Activity Assay, Mutagenesis
Journal: bioRxiv
Article Title: The EGF/EGFR-YAP1/TEAD2 axis-mediated BRAF inhibitor vemurafenib resistance upregulates STIM1 in melanoma
doi: 10.1101/2024.01.29.577717
Figure Lengend Snippet: Expression of YAP1 and STIM1 in melanoma tissues and survival analysis in melanoma patients. A. Correlation analysis of YAP1 and STIM1 mRNA level from TCGA database. B. Representative images of YAP1 and STIM1 immunohistochemical staining in melanoma. C. The correlation analysis between YAP1 and STIM1 in melanoma /Correlation analysis of YAP1 and STIM1 level in patients with melanoma/ Comparison analysis of YAP1 and STIM1 expression levels in melanoma with different Clark grade. D. Model for vemurafenib resistance induced STIM1 upregulation.
Article Snippet: The following antibodies were used in this study: mouse monoclonal GAPDH(G8795; Sigma-Aldrich, Shanghai, China), mouse monoclonal STIM1(sc-166840; Santa Cruz, Shanghai, China), rabbit polyclonal Actin(sc-1616; Santa Cruz, Shanghai, China), mouse monoclonal Raf-B(sc-5284; Santa Cruz, Shanghai, China), mouse monoclonal EGFR(sc-373746; Santa Cruz, Shanghai, China), mouse monoclonal GAPDH(sc-32233; Santa Cruz, Shanghai, China), mouse monoclonal p-ERK(sc-7383; Santa Cruz, Shanghai, China), mouse monoclonal ERK(sc-514302; Santa Cruz, Shanghai, China), mouse monoclonal p-stat3(sc-8059; Santa Cruz, Shanghai, China), rabbit polyclonal β-catenin(A01211-40; Genscript, China),
Techniques: Expressing, Immunohistochemistry, Staining, Comparison